6IBB
Crystal structure of the rat isoform of the succinate receptor SUCNR1 (GPR91) in complex with a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.220, 164.000, 63.420 |
| Unit cell angles | 90.00, 102.63, 90.00 |
Refinement procedure
| Resolution | 41.550 - 2.120 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xnv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS (BUILT 20160617) |
| Data scaling software | XSCALE (BUILT 20160617) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.550 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.763 | 2.119 |
| Number of reflections | 198484 | |
| <I/σ(I)> | 2.92 | 0.32 |
| Completeness [%] | 88.2 | 20.8 |
| Redundancy | 17.88 | 1.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293.15 | 0.1M Na-Citrate 24-30% PEG400 0.05M NaSCN 2.5% 2,5-Hexanediol 1% DMSO |
