6I9C
Structure of the OTU domain of OTULIN G281R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-08-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.260, 72.437, 93.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.316 - 1.770 |
| R-factor | 0.1632 |
| Rwork | 0.162 |
| R-free | 0.17860 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.774 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.440 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.058 | 0.840 |
| Rmeas | 0.063 | 0.913 |
| Rpim | 0.025 | 0.354 |
| Total number of observations | 189022 | |
| Number of reflections | 29060 | 1655 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 6.5 | 6.6 |
| CC(1/2) | 0.999 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 100 mM HEPES pH 7.4, 18 % (w/v) PEG 6,000, 1M LiCl |
