6I7L
Crystal structure of monomeric FICD mutant L258D complexed with MgAMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 186.358, 186.358, 77.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 93.180 - 2.320 |
R-factor | 0.21635 |
Rwork | 0.215 |
R-free | 0.25095 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0u |
RMSD bond length | 0.003 |
RMSD bond angle | 1.173 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.180 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.071 | 0.611 |
Rmeas | 0.089 | 0.771 |
Rpim | 0.054 | 0.465 |
Number of reflections | 34573 | 3351 |
<I/σ(I)> | 10.3 | 1.8 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 4.6 | 4.6 |
CC(1/2) | 0.998 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M Sodium Chloride; 10% Ethanol |