6I3Y

Crystal structure of the human mitochondrial PRELID1K58V-TRIAP1 complex with PS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 63 2 2
Unit cell lengths	126.000, 126.000, 178.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.000 - 2.980
R-factor	0.24672
Rwork	0.244
R-free	0.29892
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i3v
RMSD bond length	0.011
RMSD bond angle	1.527
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.000	3.030
High resolution limit [Å]	2.980	2.980
Number of reflections	17749	894
<I/σ(I)>	9.1
Completeness [%]	100.0	100
Redundancy	37.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	100 mM sodium cacodylate pH 6.5 40% PEG 300