6I2I
Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | PHENIX,EPU,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
