6HZZ
Structure of human D-glucuronyl C5 epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.820, 99.820, 262.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.830 - 2.520 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pw2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.830 | 2.670 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.110 | 0.730 |
| Number of reflections | 47276 | |
| <I/σ(I)> | 12.2 | 1.9 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 21.5% P2K MME, 0.1 M Na cacodylate pH 6.0) |
