6HYU
Crystal structure of DHX8 helicase bound to single stranded poly-adenine RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-11 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 66.104, 138.601, 169.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.750 - 3.220 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 3.440 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.381 | |
| Number of reflections | 25940 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.790 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% 2-Methyl-2,4-pentanediol, 100 mM Sodium Acetate, 6% DMSO |
