6HXW
structure of human CD73 in complex with antibody IPH53
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98004 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 195.347, 209.429, 65.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.840 - 2.780 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.840 | 2.930 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.122 | 0.795 |
Rmeas | 0.129 | |
Rpim | 0.041 | 0.265 |
Number of reflections | 68010 | 9400 |
<I/σ(I)> | 5.5 | 1 |
Completeness [%] | 99.5 | 95.2 |
Redundancy | 9.3 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M succinic acid pH7.0 15% (w/v) PEG 3350 |