6HX8

Tubulin-STX3451 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	105.670, 159.920, 181.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.345 - 2.402
R-factor	0.1977
Rwork	0.195
R-free	0.24000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5osk
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.093	2.660
Rmeas	0.101	2.889
Rpim	0.042	1.158
Number of reflections	115121	8408
<I/σ(I)>	16.7
Completeness [%]	100.0	100
Redundancy	6.8	6.6
CC(1/2)	0.999	0.294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	10% PEG 4K, 16% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/imidazole pH 6.7