6HQU
Humanised RadA mutant HumRadA22 in complex with a recombined BRC repeat 8-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.403, 75.473, 114.792 |
| Unit cell angles | 90.00, 97.06, 90.00 |
Refinement procedure
| Resolution | 85.870 - 1.970 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 85.870 | 85.870 | 1.972 |
| High resolution limit [Å] | 1.966 | 9.121 | 1.966 |
| Rmerge | 0.078 | 0.039 | 2.388 |
| Rmeas | 0.091 | 0.046 | 2.778 |
| Rpim | 0.046 | 0.024 | 1.404 |
| Total number of observations | 522829 | 5022 | 5548 |
| Number of reflections | 137994 | 1454 | 1441 |
| <I/σ(I)> | 7.4 | 26.3 | 0.5 |
| Completeness [%] | 99.8 | 98.3 | 100 |
| Redundancy | 3.8 | 3.5 | 3.9 |
| CC(1/2) | 0.998 | 0.997 | 0.272 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.2 M NH4Cl, 20% w/v PEG 3350 |
