6HOH

Structure of VPS34 LIR motif (S249E) bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 21 3
Unit cell lengths	119.502, 119.502, 119.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.440 - 2.250
R-factor	0.2084
Rwork	0.206
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.002
RMSD bond angle	0.422
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.443	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.080	0.980
Rmeas	0.090	1.070
Rpim	0.030	0.440
Number of reflections	27219	2654
<I/σ(I)>	10.4
Completeness [%]	99.5	99.21
Redundancy	5.8	5.9
CC(1/2)	0.990	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 200 mM magnesium formate