6HIL
X-ray structure of TEAD1(Y421H mutant) complexed with YAP(wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 40.125, 46.544, 144.337 |
Unit cell angles | 98.61, 90.85, 108.14 |
Refinement procedure
Resolution | 19.900 - 2.300 |
R-factor | 0.2437 |
Rwork | 0.242 |
R-free | 0.26740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kys |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.046 | 0.568 |
Rmeas | 0.065 | 0.804 |
Rpim | 0.100 | 0.702 |
Total number of observations | 74068 | |
Number of reflections | 41611 | |
<I/σ(I)> | 10.9 | 1.2 |
Completeness [%] | 95.5 | 95.4 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 22% (w/v) PEG3350, 0.2M Na Malonate |