6HIK

X-ray structure of TEAD4(Y429H) mutant) complexed with YAP (wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99981
Spacegroup name	P 41 21 2
Unit cell lengths	58.843, 58.843, 159.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 1.650
R-factor	0.2252
Rwork	0.225
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.006
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.079	1.010
Rmeas	0.083	1.050
Rpim		0.880
Total number of observations	449159
Number of reflections	34599
<I/σ(I)>	21	2.6
Completeness [%]	99.9	100
Redundancy	13	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	1.4M Na/K PO4