6HER

Mouse prion protein in complex with Nanobody 484

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	37.328, 74.646, 116.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.323 - 1.199
R-factor	0.1699
Rwork	0.169
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ma8
RMSD bond length	0.005
RMSD bond angle	0.781
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.323	1.260
High resolution limit [Å]	1.199	1.200
Number of reflections	96266
<I/σ(I)>	26.9
Completeness [%]	93.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris pH 8, 1.5 M ammonium sulfate