6HER
Mouse prion protein in complex with Nanobody 484
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-11-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.328, 74.646, 116.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.323 - 1.199 |
R-factor | 0.1699 |
Rwork | 0.169 |
R-free | 0.19480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ma8 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.781 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.323 | 1.260 |
High resolution limit [Å] | 1.199 | 1.200 |
Number of reflections | 96266 | |
<I/σ(I)> | 26.9 | |
Completeness [%] | 93.6 | |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris pH 8, 1.5 M ammonium sulfate |