6HEI
Structure of the catalytic domain of USP28 (insertion deleted) bound to Ubiquitin-PA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.505, 86.414, 97.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.510 - 1.640 |
R-factor | 0.1871 |
Rwork | 0.186 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nbf |
RMSD bond length | 0.007 |
RMSD bond angle | 0.997 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.049 | 0.637 |
Rmeas | 0.057 | 0.733 |
Number of reflections | 52145 | 5114 |
<I/σ(I)> | 13.5 | 2.3 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 4 | 4 |
CC(1/2) | 0.998 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 22% (w/v) PEG 3350, 300 mM potassium sodium tartrate |