6HDR
Human DYRK2 bound to Curcumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 83.642, 83.642, 148.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.300 - 2.200 |
| R-factor | 0.19248 |
| Rwork | 0.190 |
| R-free | 0.24263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4azf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.836 |
| Number of reflections | 27566 | 3930 |
| <I/σ(I)> | 15 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M sodium potassium phosphate, 20% PEG 3350 and 10% ethylene glycol |
