6HDC
Crystal structure of the potassium channel MtTMEM175 T38A variant in complex with a Nanobody-MBP fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00004 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 133.480, 133.480, 132.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.200 | 3.670 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmeas | 0.255 | 1.062 |
Rpim | 0.216 | 0.901 |
Number of reflections | 16953 | 3435 |
<I/σ(I)> | 6.5 | 1.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 146 | 144.6 |
CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400 |