6HDC

Crystal structure of the potassium channel MtTMEM175 T38A variant in complex with a Nanobody-MBP fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nanobody,Maltose/maltodextrin-binding periplasmic protein,Maltose/maltodextrin-binding periplasmic protein	polymer	486	53267.0	1	UniProt (P0AEX9) Pfam (PF01547) In PDB	Lama glama	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B	TMEM175	polymer	255	29450.9	1	UniProt (E4TN31) Pfam (PF06736) In PDB	Marivirga tractuosa DSM 4126 (Microscilla tractuosa)
3	C	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	B	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	1	Chemie (LMT)
5	B	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
6		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A: 1 - 122 (PDB: 6HDC)
A: 123 - 483 (UniProt: P0AEX9)

PDB	External Database	Details
Pro 484	-	expression tag
Gly 485	-	expression tag
Ala 486	-	expression tag

B: 2 - 247 (UniProt: E4TN31)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Ala 38	Thr 38	engineered mutation
Ala 248	-	expression tag
Leu 249	-	expression tag
Glu 250	-	expression tag
Val 251	-	expression tag
Leu 252	-	expression tag
Phe 253	-	expression tag
Gln 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82717.9
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	2
	Total formula weight	549.7
All*	Total formula weight	83610.0

*Water molecules are not included.