6HC8

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPA1 (313-339)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	86.860, 86.860, 148.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.927 - 1.900
R-factor	0.1709
Rwork	0.170
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.007
RMSD bond angle	0.766
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.860	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.100	2.027
Rmeas	0.103	2.086
Rpim	0.024	0.493
Number of reflections	45517	6544
<I/σ(I)>	13.3	1.7
Completeness [%]	100.0	100
Redundancy	17.8	17.7
CC(1/2)	0.998	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH = 7.5, 1.6 M ammonium sulfate, 6% PEG400