6HBY
HLA class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.960, 121.290, 68.960 |
| Unit cell angles | 90.00, 107.30, 90.00 |
Refinement procedure
| Resolution | 60.640 - 1.950 |
| R-factor | 0.19539 |
| Rwork | 0.193 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.866 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.640 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.913 |
| Rmeas | 0.144 | 1.295 |
| Number of reflections | 64792 | 4807 |
| <I/σ(I)> | 8.3 | 1.3 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.995 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.02 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane pH 7.5, 20 % PEG 3350 |
