6H8P
JMJD2A/ KDM4A COMPLEXED WITH NI(II), NOG AND Histone H1.4(18-32)K26me3 peptide (15-mer)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-05 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91741 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.722, 150.146, 57.601 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.440 - 1.983 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.21180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ox0 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.886 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.450 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.083 | 0.937 |
Number of reflections | 60704 | 5991 |
<I/σ(I)> | 26.5 | 2.5 |
Completeness [%] | 99.1 | 99.4 |
Redundancy | 8.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 277 | 0.1 M MIB buffer (malonic acid/imidazole/boric acid, pH 6.0) 25% w/v polyethylene glycol 1500 |