6H7J

ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND AGONIST ISOPRENALINE AND NANOBODY Nb80

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	116.727, 121.183, 129.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 2.800
R-factor	0.28637
Rwork	0.285
R-free	0.31745
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h6x 3p0g
RMSD bond length	0.008
RMSD bond angle	1.189
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0174)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.380	2.880
High resolution limit [Å]	2.780	2.780
Number of reflections	46114
<I/σ(I)>	5.1
Completeness [%]	98.5
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Hepes-NaOH pH7.5 and 21-24% PEG1500