6H41
Structure of the complex of the IL-5 inhibitory peptide AF17121 bound to the IL-5 receptor IL-5Ralpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 138.061, 138.061, 145.855 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.167 - 2.750 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.909 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 119.564 | 43.167 | 2.900 |
High resolution limit [Å] | 2.728 | 8.700 | 2.750 |
Rmerge | 0.082 | 2.804 | |
Rmeas | 0.145 | 0.087 | 2.899 |
Rpim | 0.039 | 0.028 | 0.731 |
Total number of observations | 322542 | 9582 | 48450 |
Number of reflections | 21928 | 806 | 3104 |
<I/σ(I)> | 13.3 | 29.2 | 2.3 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 14.7 | 11.9 | 15.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 0.1 M sodium acetate pH 5.0, 3% (w/v) PEG 4000, 15% - 20% (v/v) 2-methyl-2,4-pentanediol |