6H1F
Structure of the nanobody-stabilized gelsolin D187N variant (second domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0062 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.859, 46.835, 132.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.143 - 1.900 |
| R-factor | 0.2024 |
| Rwork | 0.199 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4s10 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.160 | |
| Number of reflections | 17289 | 1078 |
| <I/σ(I)> | 4.5 | 1.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.1 | 4.8 |
| CC(1/2) | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Potassium thiocyanate, 30%w/v PEG 2000 MME |
