6H1E
Crystal structure of C21orf127-TRMT112 in complex with SAH and H4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 71.231, 71.231, 130.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.000 - 1.900 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h1d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.180 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.187 | 2.740 |
| Rpim | 0.056 | 0.857 |
| Number of reflections | 27384 | |
| <I/σ(I)> | 1.2 | |
| Completeness [%] | 100.0 | 99.89 |
| Redundancy | 22.5 | 21.9 |
| CC(1/2) | 0.999 | 0.296 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 1.1-1.3 M Sodium Citrate |
