6H0P
The structure of C100A mutant of Arabidopsis thaliana UDP-apiose/UDP-xylose synthase in complex with NADH and UDP-D-glucuronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 144.564, 144.564, 130.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.440 - 3.470 |
| R-factor | 0.19806 |
| Rwork | 0.196 |
| R-free | 0.23904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h0n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 3.800 |
| High resolution limit [Å] | 3.470 | 3.470 |
| Rmerge | 0.151 | 0.800 |
| Number of reflections | 20833 | 4867 |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 8.3 | 7.9 |
| CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 0.8 M sodium phosphate, 0.8 M potassium phosphate, and 0.1 M sodium-HEPES (pH 7.5) |
