6H03

OPEN CONFORMATION OF THE MEMBRANE ATTACK COMPLEX

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
DetectorFEI FALCON II (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)5.6
Number of Particle288366
Software,EPU,CTFFIND,Flex-EM,Coot,iMODFIT,MODELLER,TEMPy,UCSF Chimera,RELION,PHENIX
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION