EM specimen
| Vitrification | false |
| Staining | false |
6H03 |
OPEN CONFORMATION OF THE MEMBRANE ATTACK COMPLEX |
This is a large structure. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.6 |
| Number of Particle | 288366 |
| Software | ,EPU,CTFFIND,Flex-EM,Coot,iMODFIT,MODELLER,TEMPy,UCSF Chimera,RELION,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
