6GVN
Tubulin:TM-3 DARPin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.100, 84.260, 81.980 |
| Unit cell angles | 90.00, 99.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 2.690 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eyp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.080 | 2.789 |
| High resolution limit [Å] | 2.690 | 2.692 |
| Number of reflections | 26956 | |
| <I/σ(I)> | 6.22 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG5000 MME, 0.1 M Mes pH 6.0, 150 mM AMMONIUM SULFATE |
