6GSM

Structure of a partial yeast 48S preinitiation complex in open conformation.

This is a non-PDB format compatible entry.

Replaces:  3JAQ

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	FEI FALCON III (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	5.15
Number of Particle	360729
Software	RELION,REFMAC,EMAN,EPU,Gctf,UCSF Chimera,Coot
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION