6GO7

TdT chimera (Loop1 of pol mu) - full DNA synapsis complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-15
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.8728
Spacegroup name	P 1 21 1
Unit cell lengths	47.000, 90.310, 65.900
Unit cell angles	90.00, 106.79, 90.00

Refinement procedure

Resolution	44.990 - 2.550
R-factor	0.192
Rwork	0.191
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jms
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.990	2.700
High resolution limit [Å]	2.550	2.550
Rmerge	0.135	0.762
Number of reflections	16488	2605
<I/σ(I)>	5.3
Completeness [%]	94.7	93.9
Redundancy	2
CC(1/2)	0.983	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	19-25% PEG 4000, 100-400 mM lithium sulfate, 100 mM tris