6GN0
Exoenzyme S from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 165.200, 168.760, 82.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 118.050 - 3.240 |
R-factor | 0.246 |
Rwork | 0.243 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c23 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.100 | 3.290 |
High resolution limit [Å] | 3.240 | 3.240 |
Rmeas | 0.505 | 2.396 |
Number of reflections | 37506 | 1875 |
<I/σ(I)> | 5 | 1.4 |
Completeness [%] | 99.8 | 96.7 |
Redundancy | 13.3 | 14 |
CC(1/2) | 0.980 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 12% PEG 3350, 4% Tacsimate |