6GMN
pVHL:EloB:EloC in complex with methyl 4H-furo[3,2-b]pyrrole-5-carboxylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 93.710, 93.710, 364.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.470 - 1.940 |
R-factor | 0.2048 |
Rwork | 0.203 |
R-free | 0.24390 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.155 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BUSTER-TNT |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmeas | 0.077 | 0.798 |
Number of reflections | 226893 | 36190 |
<I/σ(I)> | 13.75 | 2.4 |
Completeness [%] | 99.4 | 98.1 |
Redundancy | 7 | 7 |
CC(1/2) | 0.998 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | 0.1 M Na cacodylate pH 6.0, 0.2 M Mg acetate, 15% PEG3350, 5mM DTT |