6GLW

Structure of galectin-10 in complex with the Fab fragment of a Charcot-Leyden crystal solubilizing antibody, 1D11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	61.634, 89.220, 249.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.820 - 1.900
R-factor	0.169
Rwork	0.167
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lcl 5x4g
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.900	1.900
Rmeas	0.234	1.370
Number of reflections	107497	16333
<I/σ(I)>	6.72	1.3
Completeness [%]	99.1	94.6
Redundancy	6.3	6
CC(1/2)	0.991	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate 0.1 M sodium acetate pH 4.0 15% PEG 4000