6GKU

Structure of galectin-10 in complex with the Fab fragment of a Charcot-Leyden crystal solubilizing antibody, 6F5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	C 2 2 21
Unit cell lengths	59.014, 146.186, 185.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.240 - 1.910
R-factor	0.168
Rwork	0.166
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lcl 5x4g
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.910	1.910
Rmeas	0.240	2.150
Number of reflections	63019	9927
<I/σ(I)>	7.67	1
Completeness [%]	99.7	98.3
Redundancy	12.8	11.4
CC(1/2)	0.995	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.04 M calcium chloride 0.04 M sodium formate 0.1 M PIPES pH 7.0 8% PEG Smear High