6GIV
Structure of GluA2-N775S ligand-binding domain (S1S2J) in complex with glutamate and Rubidium Bromide at 1.75 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.956, 47.513, 49.810 |
Unit cell angles | 90.00, 110.16, 90.00 |
Refinement procedure
Resolution | 34.346 - 1.750 |
R-factor | 0.1578 |
Rwork | 0.156 |
R-free | 0.19040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3a |
RMSD bond length | 0.005 |
RMSD bond angle | 0.752 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.1) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.760 | 46.760 | 1.840 |
High resolution limit [Å] | 1.750 | 5.530 | 1.750 |
Rmerge | 0.040 | 0.373 | |
Rmeas | 0.089 | 0.047 | 0.451 |
Rpim | 0.038 | 0.020 | 0.203 |
Number of reflections | 27430 | 912 | 3998 |
<I/σ(I)> | 11.4 | 14.9 | 1.8 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 5 | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 279 | 18-24.4% PEG4000, 0.2-0.3 M Li2SO4, and 0.1 M cacodylate, pH 6.5 |