6GIV

Structure of GluA2-N775S ligand-binding domain (S1S2J) in complex with glutamate and Rubidium Bromide at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91976
Spacegroup name	C 1 2 1
Unit cell lengths	122.956, 47.513, 49.810
Unit cell angles	90.00, 110.16, 90.00

Refinement procedure

Resolution	34.346 - 1.750
R-factor	0.1578
Rwork	0.156
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3a
RMSD bond length	0.005
RMSD bond angle	0.752
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760	46.760	1.840
High resolution limit [Å]	1.750	5.530	1.750
Rmerge		0.040	0.373
Rmeas	0.089	0.047	0.451
Rpim	0.038	0.020	0.203
Number of reflections	27430	912	3998
<I/σ(I)>	11.4	14.9	1.8
Completeness [%]	100.0	99.9	100
Redundancy	5	4.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	279	18-24.4% PEG4000, 0.2-0.3 M Li2SO4, and 0.1 M cacodylate, pH 6.5