6GIH
Crystal Structure of CK2alpha with CAM187 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.488, 45.117, 62.889 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 20.890 - 1.960 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cu6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XPREP |
| Data scaling software | SHELXPREP (2013/3) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.750 | 35.750 | 2.000 |
| High resolution limit [Å] | 1.960 | 8.340 | 1.960 |
| Rmerge | 0.086 | 0.050 | 0.400 |
| Total number of observations | 194010 | ||
| Number of reflections | 21348 | 323 | 969 |
| <I/σ(I)> | 17.69 | ||
| Completeness [%] | 94.9 | 98.8 | 81.8 |
| Redundancy | 8.62 | 11.54 | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |
