6GID
High resolution crystal structure of substrate-free human neprilysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.424, 107.424, 112.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.032 - 1.900 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jmy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.894 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.032 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59422 | |
| <I/σ(I)> | 25.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 53.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M potassium nitrate and 20% (w/v) PEG3350 |
