6GDY

Crystal structure of 2OG oxygenase JMJD6 (aa 1-343) in complex with Fe(II) and 2OG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07206
Spacegroup name	P 1 21 1
Unit cell lengths	47.150, 96.990, 93.760
Unit cell angles	90.00, 95.84, 90.00

Refinement procedure

Resolution	42.227 - 2.040
R-factor	0.1812
Rwork	0.179
R-free	0.21620
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX ((dev_2906: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.227	9.120
High resolution limit [Å]	2.040	2.040
Rmerge	0.130	1.297
Rmeas	0.157	1.532
Rpim	0.060	0.590
Number of reflections	53266	3866
<I/σ(I)>	9.7	1.4
Completeness [%]	99.8	99.5
Redundancy	6.7	6.6
CC(1/2)	0.997	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	0.1M HEPES pH 7.8, 0.7M NaH2PO4, 0.9M KH2PO4, 3% glycerol