6GB7
Structure of H-2Db with scoop loop from tapasin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.700, 123.860, 100.110 |
| Unit cell angles | 90.00, 103.66, 90.00 |
Refinement procedure
| Resolution | 52.242 - 2.150 |
| R-factor | 0.2404 |
| Rwork | 0.239 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.658 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.280 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.113 | 0.506 |
| Number of reflections | 117221 | 5702 |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 98.4 | 97.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.8M ammonium sulfate, 0.1M Tris-HCl (pH 8) and 0.5M NaCl |
