6G5J
Secreted phospholipase A2 type X in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.962, 85.501, 103.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.760 - 1.850 |
| R-factor | 0.19568 |
| Rwork | 0.194 |
| R-free | 0.22779 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.150 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.478 |
| Number of reflections | 21588 | |
| <I/σ(I)> | 18.4 | 2 |
| Completeness [%] | 91.0 | 89.8 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 37-45% PEG400 0.1M bis-Tris pH 5.6-5.9 |
