6G3A
Crystal structure of haspin F605T mutant in complex with 5-iodotubercidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.351, 78.700, 79.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.000 - 1.430 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.17500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ouc |
RMSD bond length | 0.013 |
RMSD bond angle | 1.624 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.010 | 1.450 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.122 | |
Number of reflections | 91424 | |
<I/σ(I)> | 11.3 | 2.7 |
Completeness [%] | 99.8 | 96.9 |
Redundancy | 9.3 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 51-63% MPD and 0.1M SPG buffer, pH 6.0-6.5 |