6FZF
PPAR mutant complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.644, 123.684, 54.466 |
| Unit cell angles | 90.00, 95.31, 90.00 |
Refinement procedure
| Resolution | 17.626 - 1.950 |
| R-factor | 0.1534 |
| Rwork | 0.152 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1prg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.845 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.626 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.753 |
| Number of reflections | 50678 | |
| <I/σ(I)> | 12.42 | |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5 |
