6FYX

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse
InstrumentFEI VITROBOT MARK I

EM Data Collection

MicroscopeFEI POLARA 300
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2
DetectorFEI FALCON III (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.5
Number of Particle706713
SoftwareRELION,EMAN,EPU,,Gctf,UCSF Chimera,Coot,REFMAC
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION