6FY2
Crystal structure of a V2p-reactive RV144 vaccine-like antibody, CAP228-16H, in complex with a heterologous CAP225 V1V2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.354, 41.757, 144.524 |
| Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
| Resolution | 36.090 - 2.301 |
| R-factor | 0.1905 |
| Rwork | 0.190 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1200009079 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13.2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.194 | 1.206 |
| Rpim | 0.121 | 0.743 |
| Number of reflections | 58982 | |
| <I/σ(I)> | 7.6 | 4 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.952 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 0.1 M HEPES NaOH (pH7.5), 20% PEG8000, 0.2 M ammonium sulphate, 10% isopropanol, and cryoprotected with 20% glycerol |
