6FXT

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP-Glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	97.744, 100.472, 225.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.498 - 2.500
R-factor	0.2181
Rwork	0.216
R-free	0.24810
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.574
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.143	1.590
Rpim	0.103	1.147
Number of reflections	38604	4301
<I/σ(I)>	8.7	0.9
Completeness [%]	99.6	99.7
Redundancy	5.1	5.3
CC(1/2)	0.996	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 0.5 mM FeCl2, 0.5 mM MnCl2, 1 mM UDP-Glc