6FUT
Complement factor D in complex with the inhibitor (S)-3'-(aminomethyl)-N-(1,2,3,4-tetrahydronaphthalen-1-yl)-[1,1'-biphenyl]-3-carboxamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-12-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.225, 77.041, 84.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.050 - 1.500 |
R-factor | 0.15908 |
Rwork | 0.158 |
R-free | 0.18382 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.469 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.100 | 1.510 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.512 | |
Number of reflections | 52456 | |
<I/σ(I)> | 15.1 | 3.2 |
Completeness [%] | 98.0 | |
Redundancy | 5.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22-25% PEG3350, 100 mM HEPES pH 7.5 |