6FUF

Crystal structure of the rhodopsin-mini-Go complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	151.359, 151.359, 96.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.544 - 3.117
R-factor	0.2592
Rwork	0.257
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a4m 5g53
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.544	3.508
High resolution limit [Å]	3.020	3.020
Rmerge		5.087
Rmeas	0.368	5.172
Rpim	0.060	0.930
Number of reflections	14856	719
<I/σ(I)>	9.416	1.937
Completeness [%]	58.0	8
Redundancy	37.3	30.7
CC(1/2)	1.000	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M MES pH 5.5 10-20% PEG4000
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M MES pH 5.5 10-20% PEG4000
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M MES pH 5.5 10-20% PEG4000
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M MES pH 5.5 10-20% PEG4000
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M MES pH 5.5 10-20% PEG4000