6FUF

Crystal structure of the rhodopsin-mini-Go complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Rhodopsin	polymer	316	35800.9	1	UniProt (P02699) Pfam (PF10413) Pfam (PF00001)	Bos taurus (Bovine)
2	B (B)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	214	24317.8	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (Human)
3	C (A)	RETINAL	non-polymer		284.4	1	Chemie (RET)
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	E (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 2 - 317 (UniProt: P02699)

PDB	External Database	Details
Cys 2	Asn 2	engineered mutation
Tyr 257	Met 257	engineered mutation
Cys 282	Asp 282	engineered mutation

B: 18 - 354 (UniProt: P09471)

PDB	External Database	Details
Gly 18	Ala 18	engineered mutation
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation
-	Glu 64	deletion
-	Asp 65	deletion
-	Val 66	deletion
-	Lys 67	deletion
-	Gln 68	deletion
-	Tyr 69	deletion
-	Lys 70	deletion
-	Pro 71	deletion
-	Val 72	deletion
-	Val 73	deletion
-	Tyr 74	deletion
-	Ser 75	deletion
-	Asn 76	deletion
-	Thr 77	deletion
-	Ile 78	deletion
-	Gln 79	deletion
-	Ser 80	deletion
-	Leu 81	deletion
-	Ala 82	deletion
-	Ala 83	deletion
-	Ile 84	deletion
-	Val 85	deletion
-	Arg 86	deletion
-	Ala 87	deletion
-	Met 88	deletion
-	Asp 89	deletion
-	Thr 90	deletion
-	Leu 91	deletion
-	Gly 92	deletion
-	Ile 93	deletion
-	Glu 94	deletion
-	Tyr 95	deletion
-	Gly 96	deletion
-	Asp 97	deletion
-	Lys 98	deletion
-	Glu 99	deletion
-	Arg 100	deletion
-	Lys 101	deletion
-	Ala 102	deletion
-	Asp 103	deletion
-	Ala 104	deletion
-	Lys 105	deletion
-	Met 106	deletion
-	Val 107	deletion
-	Cys 108	deletion
-	Asp 109	deletion
-	Val 110	deletion
-	Val 111	deletion
-	Ser 112	deletion
-	Arg 113	deletion
-	Met 114	deletion
-	Glu 115	deletion
-	Asp 116	deletion
-	Thr 117	deletion
-	Glu 118	deletion
-	Pro 119	deletion
-	Phe 120	deletion
-	Ser 121	deletion
-	Ala 122	deletion
-	Glu 123	deletion
-	Leu 124	deletion
-	Leu 125	deletion
-	Ser 126	deletion
-	Ala 127	deletion
-	Met 128	deletion
-	Met 129	deletion
-	Arg 130	deletion
-	Leu 131	deletion
-	Trp 132	deletion
-	Gly 133	deletion
-	Asp 134	deletion
-	Ser 135	deletion
-	Gly 136	deletion
-	Ile 137	deletion
-	Gln 138	deletion
-	Glu 139	deletion
-	Cys 140	deletion
-	Phe 141	deletion
-	Asn 142	deletion
-	Arg 143	deletion
-	Ser 144	deletion
-	Arg 145	deletion
-	Glu 146	deletion
-	Tyr 147	deletion
-	Gln 148	deletion
-	Leu 149	deletion
-	Asn 150	deletion
-	Asp 151	deletion
-	Ser 152	deletion
-	Ala 153	deletion
-	Lys 154	deletion
-	Tyr 155	deletion
-	Tyr 156	deletion
-	Leu 157	deletion
-	Asp 158	deletion
-	Ser 159	deletion
-	Leu 160	deletion
-	Asp 161	deletion
-	Arg 162	deletion
-	Ile 163	deletion
-	Gly 164	deletion
-	Ala 165	deletion
-	Ala 166	deletion
-	Asp 167	deletion
-	Tyr 168	deletion
-	Gln 169	deletion
-	Pro 170	deletion
-	Thr 171	deletion
-	Glu 172	deletion
-	Gln 173	deletion
-	Asp 174	deletion
-	Ile 175	deletion
-	Leu 176	deletion
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Asp 250	Leu 250	engineered mutation
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60118.7
Non-Polymers*	Number of molecules	2
	Total formula weight	505.6
All*	Total formula weight	60624.4

*Water molecules are not included.