6FQJ

GluA2(flop) G724C ligand binding core dimer bound to ZK200775 at 2.50 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	86.010, 144.290, 199.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.830 - 2.500
R-factor	0.2009259541
Rwork	0.198
R-free	0.26491
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kgc
RMSD bond length	0.013
RMSD bond angle	1.274
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.830	2.340
High resolution limit [Å]	2.259	2.260
Rmeas	0.121
Rpim	0.086
Number of reflections	114724
<I/σ(I)>	6.8
Completeness [%]	99.9
Redundancy	1.9
CC(1/2)	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.2M Ammonium Chloride, 20% PEG 3350, 10microM ZK200775.