6FMF

Neuropilin-1 b1 domain in complex with EG01377; 2.8 Angstrom structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54179
Spacegroup name	P 41
Unit cell lengths	39.655, 39.655, 91.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.671 - 2.811
R-factor	0.2351
Rwork	0.233
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kex
RMSD bond length	0.004
RMSD bond angle	1.335
Data reduction software	d*TREK
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.650	2.960
High resolution limit [Å]	2.800	2.800
Rmerge	0.180	0.350
Rpim	0.075
Number of reflections	3271
<I/σ(I)>	10.9
Completeness [%]	98.1	82.2
Redundancy	12.8
CC(1/2)	0.994	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	16 % w/v PEG3350 and 200 mM ammonium chloride